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KMID : 0350519920450020491
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Journal of Catholic Medical College
1992 Volume.45 No. 2 p.491 ~ p.502
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Effect of Cytokines and Other Factors on the PreS1 and PreS2 Promoter Activities of the Hepatitis B Virus(subtype adr)
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Abstract
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Expression of PreS1/PreS2/S gene is regulated by complex interplay between regulatory sequences in the PreS1 and PreS2 promoters and many other positive and negative elements, such as viral enhancers, cis-acting element in the HBx gene, cellular
transcription factors, Although the exact mechanisms that regulate expression of surface antigen genes are not clear, cell line specificity and subtype specificity were observed by many studies which used adw and ayw subtypes, that are not common
in
Korea. The DNA fragments of the PreS1 and PreS2 promoters were amplified from the HBV DNA(subtype adr) which were isolated from a Korean HBsAg carrier by polymerase chain reaction(PCR). Oligonucleotides which contains either Hind III or Xba I
recognition sites on their 5* ends or 3* ends respectively were generated by oligonucleotide synthesizer and they were used as primers for PCR. After ligating these DNA fragments into chloramphenicol acety1 transferase(CAT) reporter gene, the
promoter
activities were analysed by transient transfection assays. The influences of cytokines, oncogenes, tumor suppressor gene, viral transactivators and transcription factor on the activities of PreS1 and PreS2 promoters were screened by CAT assays.
While
Ras, Src, E7, SV40T and AP-2 increased the PreS1 and PreS2 promoter activities, tumor necrosis factor a was shown to inhibit these promoter activities.
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KEYWORD
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